Improving cell-specific productivities (CSP) is an important goal for the optimization of biopharmaceutical processes using mammalian producers such as Chinese hamster ovary (CHO) cells. However, this requires quantitative understanding of subcellular mechanisms complementing conventional approaches of bioprocess design.
The cell culture experts Dr. Martin Schilling from Evonik Health Care and Prof. Dr. Ralf Takors from University of Stuttgart, will be investigating multiple tyrosine-containing dipeptides and show how they improve intracellular ATP availabilities. Further studying cytosolic and mitochondrial metabolite pools and 13C labeling patterns outlines the necessity to engineering NADPH supply for maximizing antibody formation. The webinar will also visit the question of whether the addition of S-adenosyl methionine (SAM) boosts CSP thanks to improved NADPH availability.